Looking for a quick way to design experiments?
Try the Workflow Configurator. A convenient tool to build experimental workflows and find products to match your needs.

ipsogen JAK2 MutaSearch Kit

采用real-time PCR对JAK2 V617F/G1849T突变进行定量检测

S_1084_5_GEN_disclaimer

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

ipsogen JAK2 MutaSearch Kit (24)

Cat. No. / ID:   673813

For 24 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, 2 Primers and Probe Mixes JAK2 wild-type and JAK2 V617F
登录 要查看您的账户定价。
仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 灵敏、准确的定量检测试剂
  • 能够检测1%临界值的阳性样本
  • 即用型解决方案,便利的工作流程

产品详情

ipsogen JAK2 MutaSearch Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对基因组DNA中的JAK2 V617F/G1849T突变进行灵敏、可靠的检测。

绩效

为确保检测的高灵敏度,ipsogen JAK2 MutaSearch Kit已经过优化,利用1%的DNA临界样本(COS)、阳性和阴性对照,检测JAK2 V617F/G1849T突变(参见 Accurate detection of JAK2 V617F mutation, 1% cut-off sample, and positive and negative controls Reliable detection of wild-type JAK2, 1% cut-off sample, and positive and negative controls)。推荐的起始样本DNA最少为25 ng。
查看图表

原理

ipsogen JAK2 MutaScreen Kits为即用型试剂盒,能够对基因组DNA中SNP导致的JAK2 V617F/G1849T体细胞突变进行检测分析(参见 JAK2 mutation site identification)。该试剂盒灵敏度高,可重复性强。ipsogen JAK2 MutaScreen Kits提供JAK2 V617F的DNA检测临界样本(COS)、DNA阳性对照(PC)和阴性对照(NC)。使用检测临界样本和对照能够实现准确的基因分型分析。
查看图表

程序

第一步是抽提总DNA,并检测其量与品质。第二步是通过real-time PCR扩增DNA。ipsogen JAK2 MutaSearch Kits能够对JAK2 V617F/G1849T突变进行灵敏的定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

应用

ipsogen JAK2 MutaSearch Kits能够对JAK2 V617F/G1849T突变进行灵敏、可靠的定量检测,仅适用于科研。

辅助数据和图表

资源

试剂盒操作手册 (1)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
//dev-homepage/applications/digital-pcr/promotions/registration/search/products?query=QIAGEN%20Plasmid%20Mini%20Kit%20(100/search3/products?query=dna/search3/products/dev-search/products?query=dna/dev-search/products/product-categories/discovery-and-translational-research/genomic-services/product-categories/discovery-and-translational-research/genomic-services?disable-wfc=true&disable-dtm=true/products/products/diagnostics-and-clinical-research/sample-processing/allprep-rnaprotein-kit/products/diagnostics-and-clinical-research/sample-processing/allprep-rnaprotein-kit/products/discovery-and-translational-research/dna-rna-purification/dna-purification/genomic-dna/blood-and-cell-culture-dna-mini-kit/products/discovery-and-translational-research/pcr-qpcr/pcr-enzymes-and-kits/hifidelity-long-range-and-other-pcr/ucp-hifidelity-pcr-kit/products/discovery-and-translational-research/lab-essentials/buffers-reagents/maxtract-high-density/products/discovery-and-translational-research/lab-essentials/buffers-reagents/maxtract-high-density?catno=129056/products/discovery-and-translational-research/lab-essentials/buffers-reagents/maxtract-high-density?catno=129065/products/diagnostics-and-clinical-research/sample-processing/allprep-rnaprotein-kit/BAD_URL/product-categories/discovery-and-translational-research/genomic-services/BAD_URL/products?cmpid=1234&intcmp=456/products?cmpid=asdf&intcmp=qwertysds-searchpromotionsknowledge-and-support/resourcesapplications/enzymes/tools-and-calculatorsapplications/enzymes/tools-and-calculators/ligation-calculator