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QIAsymphony DSP AXpH DNA Kit

从液基细胞学介质中自动分离和纯化DNA

Features

  • 预装的新型试剂卡夹,简便易用
  • 通过标准化流程获得高度的可重复性
  • 每次运行可上样多达96个样本
  • 条形编码技术,支持追踪样本和试剂

Product Details

QIAsymphony DSP AXpH DNA Kit与QIAsymphony SP核酸提取纯化分析仪配合使用,能够从液基细胞学介质中自动纯化DNA。优化的实验方案能确保生成高纯度的DNA洗脱产物,可直接用于下游应用。预装的新型试剂卡夹缩短了反应体系构建时间,使手动操作时间相应缩短。条形码读取技术支持对试剂全程跟踪。QIAsymphony SP核酸提取纯化分析仪能够胜任样本制备流程中的所有步骤。单次运行可以检测多达24批共96个样本。

Principle

QIAsymphony DSP AXpH技术结合了pH值驱动的阳离子交换层析技术的速度和效率,以及磁珠操作技术的便利(参见 Schematic of the QIAsymphony SP principle)。新型卡夹预先填装了所有用于纯化操作的相关试剂,能够由仪器自动开启,从而有助于使环境污染降至最低。
See figures

Procedure

专门设计的纯化步骤能够确保针对潜在感染性样本实施安全、可靠的操作,该流程由4个步骤所组成:裂解、结合、洗涤和洗脱(参见 QIAsymphony DSP AXpH procedure)。工作台的设置十分迅速,能够节省您宝贵的时间。在耗材抽屉中便利的装上两个试剂卡夹。试剂卡夹可以来自同一试剂盒或不同试剂盒,其能够针对同一运行反应中的96个样品实施不同的纯化流程。所使用的试剂体积与所选的样本数量相匹配,从而实现了全面的成本控制。

QIAsymphony DSP AXpH DNA Kit实验方案
实验方案 样本类型 处理的样本体积
AXpH2000_DSP PreservCyt样本 2 ml
SP2000_DSP 稀释的SurePath样本 2 ml
See figures

Applications

QIAsymphony DSP AXpH DNA Kit与QIAsymphony SP核酸提取纯化分析仪联用,能够从液基细胞学样本——例如使用SurePath(BD)或PreservCyt(Cytyc/Hologic)采集的宫颈拭样——中纯化DNA。

Supporting data and figures

Resources

Safety Data Sheets (1)

FAQ

How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?

Tissue: If no information about the expected yield is available, we recommend starting with 25 mg sample material. Depending on the yield obtained, the sample size can be increased in subsequent preparations to a maximum of 50 mg.

FFPE: Up to 4 sections, each with a thickness of up to 10 µm, or 8 sections with a thickness of up to 5 µm and a surface area of up to 250 mm2, can be combined in one preparation.

FAQ ID -2927
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