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ipsogen RUNX1-RUNX1T1 Kit

采用real-time PCR对RUNX1-RUNX1T1(或AML1-ETO)融合基因转录本进行定量检测

S_1084_5_GEN_V2

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ipsogen RUNX1-RUNX1T1 Kit (24)

Cat. No. / ID:   675013

For 24 reactions: ABL Control Gene Standards, RUNX1-RUNX1T1 Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix RUNX1-RUNX1T1 Fusion Gene
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 灵敏的定量检测试剂
  • 对与ABL对照基因表达相关的转录本进行准确定量
  • 符合EAC要求的标准化试剂,结果具有可重复性
  • 卓越的质量保证,获得ISO 13485认证
  • 即用型解决方案,便利的工作流程

产品详情

ipsogen RUNX1-RUNX1T1 Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。ipsogen RUNX1-RUNX1T1 Kit中的试剂经过优化,能够对骨髓或外周血样本中的RUNX1-RUNX1T1(或AML1-ETO)融合基因转录本相对于ABL对照基因的表达的可靠、灵敏的相对定量检测。

绩效

为确保获得高检测灵敏度,ipsogen RUNX1-RUNX1T1 Kit经过优化,能够检测宽泛的线性范围内的ABL和RUNX1-RUNX1T1融合基因转录本(参见Sensitive detection of RUNX1-RUNX1T1ABL standards)。

原理

ipsogen RUNX1-RUNX1T1 Kit为即用型试剂盒,采用real-time PCR技术,检测RUNX1-RUNX1T1融合基因转录本。该试剂盒可扩增并检测特定的RUNX1-RUNX1T1融合基因转录本(参见RUNX1-RUNX1T1 fusion gene transcriptABL control gene transcript),检测特异性、灵敏度和可重复性高。

ipsogen RUNX1-RUNX1T1 Kit为ABL基因提供3个标准品稀释液,为RUNX1-RUNX1T1融合基因提供5个标准品稀释液。试剂盒中的引物探针对的设计符合欧洲抗癌协会(Europe Against Cancer,简称EAC)的推荐。

程序

第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增cDNA。ipsogen RUNX1-RUNX1T1 Kit可对RUNX1-RUNX1T1融合基因和ABL基因转录本进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

应用

ipsogen RUNX1-RUNX1T1 Ki能够对人类细胞中RUNX1-RUNX1T1基因转录本相对于ABL对照基因的表达的可靠、灵敏的定量检测。仅适用于科研。

辅助数据和图表

资源

试剂盒操作手册 (1)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
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