QIAsymphony DSP AXpH DNA Kit

从液基细胞学介质中自动分离和纯化DNA

Features

预装的新型试剂卡夹，简便易用
通过标准化流程获得高度的可重复性
每次运行可上样多达96个样本
条形编码技术，支持追踪样本和试剂

Product Details

QIAsymphony DSP AXpH DNA Kit与QIAsymphony SP核酸提取纯化分析仪配合使用，能够从液基细胞学介质中自动纯化DNA。优化的实验方案能确保生成高纯度的DNA洗脱产物，可直接用于下游应用。预装的新型试剂卡夹缩短了反应体系构建时间，使手动操作时间相应缩短。条形码读取技术支持对试剂全程跟踪。QIAsymphony SP核酸提取纯化分析仪能够胜任样本制备流程中的所有步骤。单次运行可以检测多达24批共96个样本。

Principle

QIAsymphony DSP AXpH技术结合了pH值驱动的阳离子交换层析技术的速度和效率，以及磁珠操作技术的便利（参见）。新型卡夹预先填装了所有用于纯化操作的相关试剂，能够由仪器自动开启，从而有助于使环境污染降至最低。

See figures

QIAsymphony DSP AXpH procedure.

Procedure

专门设计的纯化步骤能够确保针对潜在感染性样本实施安全、可靠的操作，该流程由4个步骤所组成：裂解、结合、洗涤和洗脱（参见 QIAsymphony DSP AXpH procedure）。工作台的设置十分迅速，能够节省您宝贵的时间。在耗材抽屉中便利的装上两个试剂卡夹。试剂卡夹可以来自同一试剂盒或不同试剂盒，其能够针对同一运行反应中的96个样品实施不同的纯化流程。所使用的试剂体积与所选的样本数量相匹配，从而实现了全面的成本控制。

QIAsymphony DSP AXpH DNA Kit实验方案
实验方案	样本类型	处理的样本体积
AXpH2000_DSP	PreservCyt样本	2 ml
SP2000_DSP	稀释的SurePath样本	2 ml

See figures

Schematic of the QIAsymphony SP principle.

Applications

QIAsymphony DSP AXpH DNA Kit与QIAsymphony SP核酸提取纯化分析仪联用，能够从液基细胞学样本——例如使用SurePath（BD）或PreservCyt（Cytyc/Hologic）采集的宫颈拭样——中纯化DNA。

Supporting data and figures

QIAsymphony DSP AXpH procedure.

Resources

FAQ

Tissue: If no information about the expected yield is available, we recommend starting with 25 mg sample material. Depending on the yield obtained, the sample size can be increased in subsequent preparations to a maximum of 50 mg.

FFPE: Up to 4 sections, each with a thickness of up to 10 µm, or 8 sections with a thickness of up to 5 µm and a surface area of up to 250 mm2, can be combined in one preparation.

FAQ ID -2927

The minimum input sample volume when using the QIAsymphony DSP AXpH kit is 2.5mL. However, we recommend the use of a 4mL sample volume.

FAQ ID -2272

The QIAsymphony DSP AXpH DNA Kit provides DNA eluates that are ready for direct use in downstream applications, such as hybridization-based assays or enzymatic reactions. However, the eluates should not be used in PCR.

FAQ ID -2275

For short-term storage of up to 24 hours, we recommend storing purified DNA in microtiter plates at 2–8ºC. For long-term storage of over 24 hours, we recommend storing purified DNA in elution microtube racks at –20ºC.

FAQ ID -2274

The QIAsymphony DSP AXpH DNA Kit utilizes AXpH technology for automated isolation and purification of DNA from biological specimens.

FAQ ID -2273

When using the QIAsymphony DSP AXpH kit, the QIAsymphony can process approximately 160 samples in an 8 hour shift.

FAQ ID -2271