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artus Infl./H1 LC/RG RT-PCR Kit RUO

检测流感病毒A型或B型RNA和特异性检测H1N1病毒RNA(曾于2009年爆发)

Products

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artus Infl./H1 LC/RG RT-PCR Kit (24)

Cat. No. / ID:  4523003

For 24 reactions: 2 Masters (Influenza and Influenza H1), Mg Solution, 2 Positive Controls (Influenza and Influenza H1), Internal Control, Water (PCR grade)
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artus Infl./H1 LC/RG RT-PCR Kit (96)

Cat. No. / ID:  4523005

For 96 reactions: 2 Masters (Influenza and Influenza H1), Mg Solution, 2 Positive Controls (Influenza and Influenza H1), Internal Control, Water (PCR grade)

特点

  • 使用内参,具有高度可靠性
  • 灵敏的检测流感病毒A型或B型
  • 特异性检测新型H1N1流感
  • 即用型反应混合液

产品详情

artus Infl./H1 LC/RG RT-PCR Kit为即用型分子检测试剂盒,适用于real-time PCR检测。artus Infl./H1 LC/RG RT-PCR Kit的反应混合液含有所有扩增和检测已知流感A型和B型病毒所需的试剂和酶。此外,该试剂盒还含有特异性检测H1N1病毒的反应混合液。

原理

artus Infl./H1 LC/RG RT-PCR Kit的原理是使用real-time PCR技术,扩增和实时检测流感病毒基因组中一段特定序列。

每个artus Infl./H1 LC/RG RT-PCR Kit都包含流感病毒和流感H1型病毒的阳性对照。此外,该试剂盒还含有异源扩增系统,即内参,可识别PCR抑制剂。内参(IC)可在PCR中通过不同的荧光通道检测。

artus Infl./H1 LC/RG RT-PCR Kit的规格
试剂盒artus Infl./H1 LC/RG RT-PCR Kit
样品类型 痰、咽喉和鼻拭子
扩增子 流感A型病毒基因组长为43 bp的片段
流感B型病毒基因组长为94 bp的片段
流感H1型病毒(2009H1N1病毒)基因组长为80 bp的片段

程序

artus Infl./H1 LC/RG RT-PCR Kit包含对流感病毒RNA进行可靠检测的所有所需试剂,并已经过优化。只需在即用型PCR预混液和Mg溶液中加入模板RNA,即可按照试剂盒操作手册中的优化程序,在real-time PCR仪上开始反应。

推荐使用QIAamp Viral RNA Mini Kit,QIAamp MinElute Virus Spin Kit或EZ1 DSP Virus Kit进行RNA纯化。

应用

artus Infl./H1 LC/RG RT-PCR Kit可快速、灵敏的从痰、咽喉和鼻拭子中检测流感病毒RNA。该试剂盒适用于Rotor-Gene Q实时荧光定量PCR分析仪或LightCycler 1.1/1.2/1.5/2.0。

辅助数据和图表

资源

试剂盒操作手册 (1)
For use with Rotor-Gene Q instruments or LightCycler 1.1/1.2/1.5/2.0 instruments
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
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