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QIAamp DSP Virus Spin Kit

用于从人体血浆和血清中纯化病毒核酸

S_2226_RPADSPVirusSpin_202_s

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

QIAamp DSP Virus Spin Kit

Cat. No. / ID:   61704

For 50 preps: QIAamp MinElute Spin Columns, Buffers, Reagents, and Tubes
QIAamp DSP Virus Spin Kit 旨在用于体外诊断用途。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 通用的病毒核酸纯化系统
  • 高品质病毒核酸
  • 浓缩的核酸,洗脱体积为20–150 µl
  • 快速纯化,将交叉污染的风险降至最低

产品详情

QIAamp DSP Virus Spin Kit可进行基于硅胶的DNA和RNA共纯化。QIAamp DSP Virus Spin Kit可与小型离心机配合使用,或在QIAcube全自动核酸纯化仪上自动化操作。

绩效

简单的QIAamp DSP Virus Spin操作流程适合同时处理多个样本,在1小时内获得纯化的核酸。纯化的核酸可即用于下游应用或在–20°C保存。

原理

QIAamp DSP Virus Spin Kit使用离心操作流程进行基于硅胶的DNA和RNA共纯化。

程序

QIAamp DSP Virus Spin Kit操作流程包括5个步骤:裂解、结合、洗涤、干燥离心和洗脱。此试剂盒提供快速、可靠的病毒DNA和RNA纯化,将交叉污染风险降到最低(参见" Procedure")。纯化流程可在QIAcube全自动核酸纯化仪上完全自动化。如果使用QIAamp DSP Virus Spin Kit在QIAcube全自动核酸纯化仪上自动操作,由于自动移液时的体积损失、蒸发和额外的试剂消耗,仪器处理的样品数可能少于50个。但使用QIAamp DSP Virus Spin Kit手工操作,可进行50次样品制备。
查看图表

应用

QIAamp DSP Virus Spin Kit可共纯化人体血浆和血清中的RNA和DNA。

辅助数据和图表

资源

试剂盒操作手册 (2)
For the EU IVDR 2017/746 compliant kit (Kit Version 2)

June 2022
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
产品介绍与指南 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

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