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PyroMark Buffers and Solutions
采用焦磷酸测序技术,优化模板DNA的制备和分析
采用焦磷酸测序技术,优化模板DNA的制备和分析
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Cat. No. / ID: 979007
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
使用PyroMark真空工作站可快速、直接的从PCR扩增子生成焦磷酸测序模板,这些缓冲液和溶液专为此而设计。PyroMark Binding Buffer可促进亚硫酸氢盐转化后的PCR扩增子与覆盖链酶亲和素的琼脂糖凝胶微球结合。随后将这些微球固定在工作站的真空仪器上。PyroMark Denaturation Solution可温和的从作为焦磷酸测序模板的生物素标记链上分离其互补链,PyroMark Wash Buffer确保只有生物素标记的单链DNA结合在真空容器中。最后,当单链DNA释放到含焦磷酸测序引物的反应容器时,PyroMark Annealing Buffer可加强引物和模板的结合。