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artus Aspergillus diff. RG PCR Kit RUO

用于扩增并区分Aspergillus spp. DNA

S_1084_5_GEN_V2

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artus Aspergillus diff. RG PCR Kit (24) RUO

Cat. No. / ID:   4640203

For 24 reactions: Master, Magnesium Solution, Positive Control, Internal Control, Water (PCR grade)
€1,484.00
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 使用内参,可靠性极高
  • 灵敏检测Aspergillus spp.
  • 区分Aspergillus fumigatus/terreus/flavus DNA
  • 即用型反应预混液

产品详情

artus Aspergillus diff. RG PCR Kit为即用型real-time PCR分子检测试剂盒。该试剂盒提供的反应预混液包含所有扩增、检测和区分全血中Aspergillus fumigatus/terreus/flavus DNA所需的酶和试剂。

绩效

通过概率分析,得出artus Aspergillus diff. RG PCR Kit对阳性样本的检出率(不包含样本制备)为95%。对所有3个靶基因的分析灵敏度均小于或等于10拷贝/µl。
artus Aspergillus diff. RG PCR Kit的性能
试剂盒 artus Aspergillus diff. RG PCR Kit
样本类型 全血
分析灵敏度 小于等于10拷贝/µl
特异性 检测Aspergillus fumigatus/terreus/flavus DNA;与Aspergillus niger/ustus/versicolor DNA无交叉反应

原理

artus Aspergillus RG PCR Kit采用real-time PCR技术,扩增并同时检测Aspergillus fumigatus/terreus/flavus基因组的一段特定序列。该试剂盒特异性高,灵敏性强。

试剂盒提供A. fumigatus/terreus/flavus DNA的阳性对照,还提供外源性扩增对照,以检测是否存在PCR抑制。通过PCR的另外一个荧光信号通道检测内参(IC)。
artus Aspergillus diff. RG PCR Kit的规格
试剂盒 artus Aspergillus diff. RG PCR Kit
样本类型 全血
扩增子 A. fumigatus/terreus/flavus基因组的一段110 bp序列

程序

artus Aspergillus diff. RG PCR Kit提供可靠检测并区分Aspergillus fumigatus/terreus/flavus DNA所需的所有优化试剂。只需将模板DNA加入即用型PCR预混液和Mg溶液中,在适用的real-time PCR仪上,根据试剂盒操作手册选择优化的扩增程序,开始实验即可。我们推荐使用QIAamp UCP PurePathogen Blood Kit进行DNA纯化。

应用

artus Aspergillus diff. RG PCR Kit能够快速、灵敏的检测全血中的Aspergillus spp. DNA。该试剂盒通过特异性探针,能够分辨A. fumigatus/terreus/flavus DNA。

资源

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
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