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artus HAV TM RT-PCR Kit v2.0 RUO

使用real-time PCR技术定量检测甲型肝炎病毒RNA

S_1084_5_GEN_V2

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artus HAV TM RT-PCR Kit v2.0 (24) RUO

Cat. No. / ID:   4516103

For 24 reactions: 2 Masters, 4 Quantitation Standards, Internal Control, Water (PCR grade)
€1,785.00
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 使用内参,具有高度可靠性
  • 可灵敏检测0.1 IU/μl的RNA
  • 提供4个标准品,可进行准确的定量检测

产品详情

artus HAV TM RT-PCR Kit v2.0是使用real-time PCR技术的即用型分子检测试剂盒,适用于ABI PRISM 7000、7700和7900HT Sequence Detection Systems。该试剂盒可对人类血清和血浆样本中的甲型肝炎病毒RNA进行快速、可靠、准确定量检测;试剂盒含有所有所需试剂,并已经过优化。

绩效

为确保高灵敏度,artus HAV TM RT-PCR Kit v2.0已经过优化,可检测到低拷贝数的甲型肝炎病毒RNA。该试剂盒在ABI PRISM 7000 SDS上使用的分析灵敏度为0.2 IU/μl,在ABI PRISM 7700 SDS和ABI PRISM 7900HT SDS上的灵敏度为0.1 IU/μl。

为确保可靠的特异性,已经用artus HAV TM RT-PCR Kit v2.0检测了甲型肝炎病毒的多个株系,包括相关的基因型(IA、IB、II、IIIA和VII)和病原体。

artus HAV TM RT-PCR Kit v2.0的性能
试剂盒artus HAV TM RT-PCR Kit v2.0
样本类型 血浆或血清
分析灵敏度 0.2 IU/µl(ABI PRISM 7000 SDS);0.1 IU/µl(ABI PRISM 7700 SDS和7900HT SDS)
特异性 甲型肝炎病毒,包括IA、IB、II、IIIA和VII基因型

原理

artus HAV TM RT-PCR Kit v2.0的原理是使用real-time PCR技术,扩增和检测甲型肝炎病毒基因组中一段高度保守的序列。该试剂盒特异性强、灵敏度高,且重复性好。

artus HAV TM RT-PCR Kit v2.0包含4个甲型肝炎病毒标准品(参见" Reliable quantitation of HAV load")。使用这些标准品可确保准确定量病毒。此外,该试剂盒还含有异源扩增系统,即内参,可识别PCR抑制剂。内参(IC)可在PCR中通过不同的荧光通道检测,而不会降低甲型肝炎病毒RT-PCR的检测灵敏度。

artus HAV TM RT-PCR Kit v2.0的规格
试剂盒 artus HAV TM RT-PCR Kit v2.0
样本类型 血清或血浆
扩增子 甲型肝炎病毒基因组的一段长为90 bp的序列
查看图表

程序

artus HAV TM RT-PCR Kit v2.0包含对甲型肝炎病毒RNA进行可靠定量检测的所有所需试剂,并已经过优化。只需在即用型PCR预混液中加入模板RNA,即可按照试剂盒操作手册中的优化程序,在real-time PCR仪上开始反应。

推荐使用QIAamp UltraSens Virus Kit、QIAamp Viral RNA Mini Kit、QIAamp DSP Virus Kit或QIAamp DNA Stool Mini Kit进行RNA纯化。

应用

artus HAV TM RT-PCR Kit v2.0可快速、灵敏的检测血浆和血清中的甲型肝炎病毒RNA。该试剂盒可用于ABI PRISM 7000、7700和7900HT Sequence Detection Systems。

辅助数据和图表

Specifications

FeaturesSpecifications
Quantitative/qualitativeQuantitative
Recommended sample prepDepending on the starting material and sensitivity needs: QIAamp Ultrasens Virus Kit, QIAamp Viral RNA Mini Kit, QIAamp DSP Virus Kit, QIAamp DNA Stool Mini Kit
RUO/CE/ASRRUO
Thermal cyclerABI PRISM 7000, 7700, 7900HT SDS
Sample typePlasma, serum, fecal suspensions
What detectedHepatitis A Virus RNA

资源

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (1)
For use with the ABI PRISM 7000, 7700 and 7900HT Sequence Detection Systems
Safety Data Sheets (1)
Certificates of Analysis (1)
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