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Custom qBiomarker Somatic Mutation PCR Arrays

用于快速、准确地分析自定义的一组基因的体细胞DNA突变状态

S_1084_5_GEN_V2
在 GeneGlobe 配置
寻找或定制设计合适的靶标特异性检测和组合,以研究您感兴趣的生物靶标。

Custom qBiomarker Somatic Mutation PCR Arrays

Cat. No. / ID:   337022

PCR plate with a custom panel of mutation assays
在 GeneGlobe 配置 要查看价格
Custom qBiomarker Somatic Mutation PCR Arrays 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。
在 GeneGlobe 配置
寻找或定制设计合适的靶标特异性检测和组合,以研究您感兴趣的生物靶标。

特点

  • 分析自定义的一组基因的体细胞突变状态
  • 简单的real-time PCR操作流程
  • 灵敏度高,动态范围宽
  • 可在多数PCR仪上常规使用
  • 含有预混液

产品详情

Custom qBiomarker Somatic Mutation PCR Arrays是快速、准确分析对自定义的一组基因的体细胞突变状态的研究工具。

绩效

qBiomarker Somatic Mutation PCR Arrays具有很高的灵敏性,甚至能检测福尔马林固定、石蜡包埋(FFPE)的样本(参见 " High degree of sensitivity"和" Sensitivity of qBiomarker Somatic Mutation PCR Arrays with FFPE samples")。

qBiomarker Somatic Mutation PCR Arrays可用于检测细胞系或研究样本的突变,对于毒理学、药物研发和癌症研究都十分重要(参见" Profiling of common cancer cell lines for somatic mutation status")。

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原理

Real-time PCR是检测DNA突变的灵敏、可靠的方法。通过将等位基因特异性扩增和水解探针检测相结合,qBiomarker Somatic Mutation real-time PCR assays可用于检测在野生型基因组DNA背景下低至1%的体细胞突变。Amplification Refractory Mutation System (ARMS)技术可进行等位基因特异性扩增,这种技术基于Taq聚合酶对PCR引物3’端匹配与错配的识别(参见" Principle of ARMS technology")。
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程序

将DNA模板和即用型PCR预混液简单混合,等体积加入到相同孔板的各个孔中,然后运行real-time PCR循环程序即可。qBiomarker Somatic Mutation PCR Arrays与所有的ABI、Bio-Rad、Eppendorf、Roche和Stratagene PCR仪兼容。

qBiomarker Somatic Mutation PCR Arrays有96孔板和384孔板两种规格,可用于检测疾病或通路相关的突变,而且含有可用于标准化基因拷贝数的对照。qBiomarker Somatic Mutation PCR Array也包含常规PCR的对照因子。

易于使用的数据分析

可使用Excel数据分析模板对数据进行分析。数据分析基于∆∆CT法或Average ∆CT法。

该芯片有以下规格,每种规格已包括预混液:

qBiomarker Somatic Mutation PCR Array Format A: Fluoroscein, 96-well; for Bio-Rad iCycler, iQ5, MyiQ, and MyiQ2 instruments
qBiomarker Somatic Mutation PCR Array Format A: ROX, 96-well; for ABI Standard 96-well Blocks (5700, 7000, 7300, 7500, 7900HT, ViiA 7); Bio-Rad Chromo 4 (MJ Research); Stratagene Mx3005p, Mx3000p; Eppendorf ep realplex 2/2S, and 4/4S instruments
qBiomarker Somatic Mutation PCR Array Format C: ROX, 96-well; for ABI 7500 FAST 96-well Block, 7900HT FAST 96-Well Block, StepOnePlus, and ViiA 7 FAST 96-well Block instruments
qBiomarker Somatic Mutation PCR Array Format D: ROX, 96-well; for Bio-Rad CFX96, Opticon and Opticon 2 (MJ Research); Stratagene Mx4000 instruments
qBiomarker Somatic Mutation PCR Array Format E: ROX, 384-well; for ABI 7900HT 384-well Block, ViiA 7 384-well Block; Bio-Rad CFX384 instruments
qBiomarker Somatic Mutation PCR Array Format F: ROX, 96-well; for Roche LightCycler 480 96-well Block instruments
qBiomarker Somatic Mutation PCR Array Format G : ROX, 384-well; for Roche LightCycler 480 96-well Block instruments

应用

Custom qBiomarker Somatic Mutation PCR Arrays非常适合对自定义的一组基因的体细胞突变状态进行快速、可靠的分析。

辅助数据和图表

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