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GeneRead qPCR SYBR® Green Mastermix

与GeneRead Library Quant System配套使用

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GeneRead qPCR SYBR Green/Fluor Mastermix (2)

Cat. No. / ID:   180830

Two (2) tubes each containing 1.35 ml of 2x solution, sufficient for 100 standard 25 µl reactions
JP¥30,000
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referencedye
Fluorescein
No dye
ROX
GeneRead qPCR SYBR® Green Mastermix适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 产物特异性高,引物二聚体较少
  • 混合液中含有所有所需试剂
  • 可提供带有ROX染料或荧光素的不同规格

Product Details

GeneRead qPCR SYBR Green Mastermixes包含SYBR Green real-time PCR所需的所有试剂和缓冲液,经过优化,适用于各种real-time PCR仪。所有预混液都含有real-time PCR缓冲液、优质的HotStart DNA Taq Polymerase、核苷酸、和参考染料(ROX染料或荧光素),如果需要,可用于特定仪器的光学校准。只需在PCR管中加入预混液、模板和引物,即可开始反应。经过化学修饰的严谨的热启动酶能够防止引物二聚体和非特异性结合产物的形成,进而确保准确的反应结果。

Principle

GeneRead qPCR SYBR Green Mastermixes含有real-time PCR缓冲液、高品质HotStart DNA Taq聚合酶、核苷酸、和SYBR Green染料。预混液中含有荧光素、或ROX染料,用于实验优化。经过化学修饰和严格质控的HotStart酶可避免引物二聚体和非特异性产物生成,确保获得准确的反应结果。

含有荧光素的预混液
GeneRead qPCR SYBR Green Fluor Mastermix十分适用于基于SYBR Green技术并且以荧光素作为参比染料的real-time PCR仪器,包括Bio-Rad iCycler、iQ5、MyiQ和MyiQ2。

含有ROX染料的预混液
GeneRead qPCR SYBR Green ROX Mastermix十分适用于基于SYBR Green技术并且以ROX染料作为参比染料的real-time PCR仪器,包括Applied Biosystems 5700、7000、7300、7500(标准和快速模式)、7700、7900HT(标准和快速96孔板、384孔板)、StepOnePlus、ViiA 7(标准和快速96孔板、384孔板)、Eppendorf Mastercycler ep realplex带有或不带ROX filter set、Stratagene models Mx3000P、Mx3005P、Mx4000和Takara TP-800。

GeneRead qPCR SYBR Green ROX FAST Mastermix
GeneRead qPCR SYBR Green ROX FAST Mastermix专为基于SYBR Green技术的快速real-time PCR优化,不会影响特异性和灵敏度。qBiomarker SYBR Green ROX FAST Mastermix高度适用于QIAGEN Rotor-Gene Q实时荧光定量PCR分析仪、Rotor-Gene 6000 (Corbett)、ABI、Eppendorf、Agilent/Stratagene和TaKaRa的PCR仪。

Applications

GeneRead qPCR SYBR Green Mastermixes十分适用于基于SYBR Green技术的real-time应用,专为GeneRead DNAseq Library Quant Array和GeneRead Library Quant System设计。

Resources

Brochures & Guides (1)
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