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QIAprep High-Throughput Plates

用于自动纯化最多达 20 µg/孔分子生物学级质粒 DNA

S_2402_TurboFilter96

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

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TurboFilter 96 Plates (96)

Cat. No. / ID:   120025

TurboFilter 96 Plates (96)
NOK 109,190.00
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Plate
TurboFilter 96 Plates
QIAprep 96 Plates
QIAprep High-Throughput Plates 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 精简的纯化
  • 在 70 分钟内可纯化得到即用型质粒 DNA
  • 重复产出分子生物学级质粒 DNA

产品详情

QIAprep 96 Turbo Plates 和 TurboFilter 96 Plates 设计用于分离最多达 20 µg 高纯度质粒或柯斯质粒 DNA,以用于常规分子生物学应用,如测序、克隆和 PCR。QIAprep 96 Turbo 程序完全以 96 孔格式运行,从而将样本处理量降至最少。

绩效

QIAprep 96 Plates 与 TurboFilter 96 plates 配合使用,可自动纯化最多达 20 µg/孔分子生物学级质粒 DNA。

原理

QIAprep 96 plates 含独特的硅胶膜,在高浓度离液盐中可结合多达 20 μg DNA,然后用少量低盐缓冲液洗脱。QIAprep 膜技术去除了耗时的苯酚/氯仿提取和乙醇沉淀,并解决了树脂松散和稀浆带来的问题与不便。从 QIAprep 96 plates 洗脱得到的纯质粒 DNA 可直接使用,无需沉淀、浓缩或脱盐。

TurboFilter 96 plates 是非对称过滤装置,专门设计用于快速澄清在高浓度离液盐下产生的细菌裂解物。粗制的细菌裂解物可通过 TurboFilter 96 plate 进行真空过滤,无需离心。将不含颗粒的裂解物直接流入 QIAprep 96 plate 的孔中,可随时纯化和洗脱。TurboFilter 技术简化了 QIAprep 质粒纯化,能让程序在 BioRobot 8000 和通用系统上完全自动化运行。

程序

使用 QIAprep High-Throughput Plates 进行质粒纯化只需简单的结合-洗涤-洗脱程序。首先,裂解细菌培养基,裂解物通过 TurboFilter 板过滤澄清。将澄清后的裂解物直接流入 QIAprep 96 plate 的孔中,让质粒 DNA 吸附到硅胶膜上。洗去杂质后,用少量洗脱缓冲液或水洗脱,得到纯 DNA。

应用

QIAprep 96 Turbo Plates 与 TurboFilter 96 Plates 配合使用,可重复提取高纯度 DNA,以适合用于大多数应用,包括:

  • 测序
  • 限制性酶切
  • 筛选
  • 连接
  • 转化

资源

Safety Data Sheets (1)
Certificates of Analysis (1)
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