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ipsogen BCR-ABL1 mbcr Kit

采用real-time PCR对BCR-ABL mbcr e1a2转录本进行定量检测

S_1084_5_GEN_disclaimer

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ipsogen BCR-ABL1 mbcr Kit (24)

Cat. No. / ID:   670013

For 24 reactions: ABL Control Gene Standards, BCR-ABL mbcr Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL mbcr Fusion Gene
仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 灵敏、准确的定量检测试剂
  • 符合EAC要求的标准化试剂,结果具有可重复性
  • 即用型解决方案,便利的工作流程

产品详情

ipsogen BCR-ABL1 mbcr Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对BCR-ABL mbcr p190 e1a2转录本在总RNA中相对于ABL对照基因的表达进行可靠、灵敏的定量检测。

绩效

为确保高灵敏度,ipsogen BCR-ABL1 mbcr Kit已经过优化,使用预稀释的质粒标准品和引物探针混合液,检测BCR-ABL mbcr p190 e1a2和ABL转录本(参见 Accurate detection of BCR-ABL mbcr plasmid standards Reliable detection of ABL plasmid standards)。
查看图表

原理

ipsogen BCR-ABL1 mbcr Kit为即用型试剂盒,采用real-time PCR技术,检测BCR-ABL mbcr p190 e1a2转录本。该试剂盒可扩增并检测总RNA中相对于ABL对照基因表达的特定BCR-ABL mbcr p190 e1a2转录本(参见 BCR-ABL mbcr fusion gene transcript ABL control gene transcript)。该试剂盒灵敏度高,特异性强,可重复性好。ipsogen BCR-ABL1 mbcr Kit为BCR-ABL mbcr提供5个标准品稀释液,为ABL提供3个标准品稀释液。使用标准品能够实现准确的定量检测。BCR-ABL mbcr定量技术已根据欧洲抗癌协会(EAC)的推荐进行了标准化,ipsogen BCR-ABL1 mbcr Kit采用经验证的技术来校准结果。
查看图表

程序

第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增cDNA。ipsogen BCR-ABL1 mbcr Kit可对BCR-ABL mbcr p190 e1a2和ABL基因转录本进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

应用

ipsogen BCR-ABL1 mbcr Kit能够对BCR-ABL mbcr p190 e1a2转录本相对于ABL对照基因的表达进行可靠、灵敏的定量检测。仅适用于科研。

辅助数据和图表

资源

试剂盒操作手册 (1)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
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