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ipsogen Leukemia
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ipsogen Leukemia
ipsogen
RT Kit
ipsogen
RT Kit
用于总RNA的逆转录实验,仅适用于科研
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ipsogen
RT Kit (33)
Cat. No. / ID:
679913
For 33 reactions: Reverse transcriptase, 5x RT buffer, dNTP mix, Random primers, RNase Inhibitor, DTT
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
✓ 快速可靠的(再)订购
特点
用于逆转录的高品质试剂
用于逆转录的可靠试剂
进行准确、可重复性的逆转录
产品详情
ipsogen
RT Kit仅适用于科研,不可用于诊断。该试剂盒中的试剂经过优化,能够在Rotor-Gene Q实时荧光定量PCR分析仪或其他PCR仪上准确、可靠的进行逆转录实验。该试剂盒适用于将总RNA逆转录为cDNA,用于下游的real-time PCR应用。
原理
ipsogen
RT Kit是即用型试剂盒,可通过逆转录PCR将总RNA逆转录为cDNA。使用该试剂盒进行的逆转录实验具有准确、可重复性高的特点。逆转录酶是具有3个不同酶活性的多功能酶,包括依赖于RNA的DNA聚合酶活性、依赖于杂交的核糖核酸外切酶活性和依赖于DNA的DNA聚合酶活性。在体内,这三种酶活性共同作用,能够将单链RNA逆转录为双链DNA。依赖于RNA的DNA聚合酶活性(逆转录)可实现RNA模板到cDNA的逆转录。因此能够合成用于克隆、PCR或测序的cDNA。
程序
第一步是抽提总RNA,并检测其量与品质。第二步是使用
ipsogen
RT Kit将总RNA逆转录为cDNA。
ipsogen
RT Kit可实现总RNA的准确、可靠的逆转录。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。
应用
ipsogen RT Kit可实现总RNA的准确、可靠的逆转录。仅适用于科研。
资源
试剂盒操作手册 (1)
(EN) - ipsogen RT Kit Handbook RUO
EN
PDF
(241KB)
安全数据表 (1)
Safety Data Sheets
EN
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Safety Data Sheets
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Certificates of Analysis (1)
Certificates of Analysis
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