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ipsogen WT1 ProfileQuant Kit

采用real-time PCR对Wilm瘤(WT1)基因转录本进行定量检测

S_1084_5_GEN_V2

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ipsogen WT1 ProfileQuant Kit (24)

Cat. No. / ID:   676913

For 24 reactions: ABL Control Gene Standards, WT1 (exon 1-2) Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix WT1
仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 灵敏、准确的定量检测试剂
  • 得到欧洲白血病网络(ELN)联合会专家的优化
  • 即用型解决方案,便利的工作流程

产品详情

ipsogen WT1 ProfileQuant Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对Wilm瘤(WT1)基因转录本在总RNA中相对于ABL对照基因的表达进行可靠、灵敏的定量检测。

绩效

为确保高灵敏度,ipsogen WT1 ProfileQuant Kit已经过优化,使用预稀释的质粒标准品和引物探针混合液,检测Wilm瘤(WT1)基因转录本(参见 Accurate detection of Wilm's tumor plasmid standards Reliable detection of ABL plasmid standards)。
查看图表

原理

ipsogen WT1 ProfileQuant Kit为即用型试剂盒,采用real-time PCR技术,检测Wilm瘤(WT1)基因转录本。该试剂盒能够进行Wilm瘤(WT1)基因转录本在总RNA中相对于ABL对照基因表达的相对定量。该试剂盒灵敏度高,特异性强,可重复性好。ipsogen WT1 ProfileQuant Kit含有特异性的质粒和引物探针混合液,用于定量WT1和ABL基因(参见WT1 gene transcript 和ABL control gene transcript)。该试剂盒包含Wilm瘤(WT1)基因的5个标准品稀释液和ABL基因的3个标准品稀释液使用标准品能够实现准确的定量检测。试剂盒中的试剂在欧洲白血病网络(ELN)联合会专家的共同研究中得以验证。

程序

第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增cDNA。ipsogen WT1 ProfileQuant Kit可对Wilm瘤(WT1)和ABL基因转录本进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

应用

ipsogen WT1 ProfileQuant Kit能够对Wilm瘤(WT1)基因转录本相对于ABL对照基因的表达进行可靠、灵敏的定量检测。仅适用于科研。

辅助数据和图表

资源

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (1)
For Research Use Only. Not for use in diagnostic procedures.
Safety Data Sheets (1)
Certificates of Analysis (1)
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