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MagAttract 96 DNA Plant Core Kit

用于从植物组织中以高通量手动或自动高效纯化细胞总 DNA

S_1084_5_GEN_V2

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

MagAttract 96 DNA Plant Core Kit (24)

Cat. No. / ID:   67163

用于 24 x 96 次制备的 MagAttract 悬浮液和缓冲液
PLN 22,497.00
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KitBuffer
MagAttract 96 DNA Plant Core Kit
Buffer RPW
MagAttract 96 DNA Plant Core Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 经济实惠的解决方案
  • 以可重现的方式生产即用型纯 DNA
  • 简单、快速且可靠的操作流程
  • 初步评估手动方案

产品详情

MagAttract 96 DNA Plant Core Kit 将硅胶基质 DNA 纯化的高效性与磁微粒的便捷操作相结合,采用手动、半自动或自动 96 孔板格式。

绩效

使用 MagAttract 96 DNA Plant Core Kit 纯化
格式: 96 孔样本板
样本来源: 植物组织
新鲜或冷冻植物起始材料: 10–100 mg 
干燥或冻干植物起始材料: 10–30 mg
典型产量: 1–15 µg
洗脱体积: 100 µl

纯化的 DNA 已成功用于 PCR 和 real-time PCR(见图“ 成功的 PCR 分析”和“ 可靠的 real-time PCR 分析”)。

查看图表

原理

MagAttract 96 DNA Plant Kit 专为从植物组织全自动、高通量制备基因组和叶绿体 DNA 而设计。成熟可靠的 MagAttract 磁微粒技术与 BioRobot Plant Science 系统(不再提供)相结合,可实现高效、可重现的 DNA 纯化(见图“ 可重现的产量”)。
查看图表

程序

使用 TissueLyser 等匀浆器对植物样本进行机械破碎。将所得粉末在裂解缓冲液中重新悬浮、充分混合并离心。将裂解物转移到含有 MagAttract 磁微粒的 96 孔微孔板上,基因组 DNA 会选择性地与微粒表面结合(见“ MagAttract 96 DNA Plant Core 操作流程”)。经过清洗,用低盐缓冲液洗脱纯基因组 DNA 后,即可将其用于下游应用。MagAttract 96 DNA Plant Kit 专为配合 BioRobot Plant Science 系统进行全自动 DNA 纯化而设计。QIAGEN 还提供使用 BioRobot RapidPlate 工作站的自动方案和用于评估的手动方案。
查看图表

应用

MagAttract 96 DNA Plant Kit 能从多种植物中以高产量提取 DNA。纯化的 DNA 大小可达 40 kb,以 23 kb 的片段为主,可随时用于下游应用,包括:

  • PCR
  • Real-time PCR
  • 微卫星分析
  • SNP 基因分型

辅助数据和图表

资源

试剂盒操作手册 (1)
For efficient high-throughput isolation of DNA from plant tissue 
安全数据表 (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
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