Looking for a quick way to design experiments?
Try the Workflow Configurator. A convenient tool to build experimental workflows and find products to match your needs.

artus Aspergillus diff. RG PCR Kit RUO

用于扩增并区分Aspergillus spp. DNA

S_1084_5_GEN_V2

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

artus Aspergillus diff. RG PCR Kit (24) RUO

Cat. No. / ID:   4640203

For 24 reactions: Master, Magnesium Solution, Positive Control, Internal Control, Water (PCR grade)
登录 要查看您的账户定价。
仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 使用内参,可靠性极高
  • 灵敏检测Aspergillus spp.
  • 区分Aspergillus fumigatus/terreus/flavus DNA
  • 即用型反应预混液

产品详情

artus Aspergillus diff. RG PCR Kit为即用型real-time PCR分子检测试剂盒。该试剂盒提供的反应预混液包含所有扩增、检测和区分全血中Aspergillus fumigatus/terreus/flavus DNA所需的酶和试剂。

绩效

通过概率分析,得出artus Aspergillus diff. RG PCR Kit对阳性样本的检出率(不包含样本制备)为95%。对所有3个靶基因的分析灵敏度均小于或等于10拷贝/µl。
artus Aspergillus diff. RG PCR Kit的性能
试剂盒 artus Aspergillus diff. RG PCR Kit
样本类型 全血
分析灵敏度 小于等于10拷贝/µl
特异性 检测Aspergillus fumigatus/terreus/flavus DNA;与Aspergillus niger/ustus/versicolor DNA无交叉反应

原理

artus Aspergillus RG PCR Kit采用real-time PCR技术,扩增并同时检测Aspergillus fumigatus/terreus/flavus基因组的一段特定序列。该试剂盒特异性高,灵敏性强。

试剂盒提供A. fumigatus/terreus/flavus DNA的阳性对照,还提供外源性扩增对照,以检测是否存在PCR抑制。通过PCR的另外一个荧光信号通道检测内参(IC)。
artus Aspergillus diff. RG PCR Kit的规格
试剂盒 artus Aspergillus diff. RG PCR Kit
样本类型 全血
扩增子 A. fumigatus/terreus/flavus基因组的一段110 bp序列

程序

artus Aspergillus diff. RG PCR Kit提供可靠检测并区分Aspergillus fumigatus/terreus/flavus DNA所需的所有优化试剂。只需将模板DNA加入即用型PCR预混液和Mg溶液中,在适用的real-time PCR仪上,根据试剂盒操作手册选择优化的扩增程序,开始实验即可。我们推荐使用QIAamp UCP PurePathogen Blood Kit进行DNA纯化。

应用

artus Aspergillus diff. RG PCR Kit能够快速、灵敏的检测全血中的Aspergillus spp. DNA。该试剂盒通过特异性探针,能够分辨A. fumigatus/terreus/flavus DNA。

资源

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
//dev-homepage/applications/digital-pcr/promotions/registration/search/products?query=QIAGEN%20Plasmid%20Mini%20Kit%20(100/search3/products?query=dna/search3/products/dev-search/products?query=dna/dev-search/products/product-categories/discovery-and-translational-research/genomic-services/product-categories/discovery-and-translational-research/genomic-services?disable-wfc=true&disable-dtm=true/products/products/diagnostics-and-clinical-research/sample-processing/allprep-rnaprotein-kit/products/diagnostics-and-clinical-research/sample-processing/allprep-rnaprotein-kit/products/discovery-and-translational-research/dna-rna-purification/dna-purification/genomic-dna/blood-and-cell-culture-dna-mini-kit/products/discovery-and-translational-research/pcr-qpcr/pcr-enzymes-and-kits/hifidelity-long-range-and-other-pcr/ucp-hifidelity-pcr-kit/products/discovery-and-translational-research/lab-essentials/buffers-reagents/maxtract-high-density/products/discovery-and-translational-research/lab-essentials/buffers-reagents/maxtract-high-density?catno=129056/products/discovery-and-translational-research/lab-essentials/buffers-reagents/maxtract-high-density?catno=129065/products/diagnostics-and-clinical-research/sample-processing/allprep-rnaprotein-kit/BAD_URL/product-categories/discovery-and-translational-research/genomic-services/BAD_URL/products?cmpid=1234&intcmp=456/products?cmpid=asdf&intcmp=qwertysds-searchpromotionsknowledge-and-support/resourcesapplications/enzymes/tools-and-calculatorsapplications/enzymes/tools-and-calculators/ligation-calculator