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QIAamp DSP DNA Mini Kit

用于纯化基因组、线粒体、细菌、寄生虫或病毒DNA

S_1084_5_GEN_V2

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

QIAamp DSP DNA Mini Kit (50)

Cat. No. / ID:   61304

For 50 DNA preps: QIAamp Mini Spin Columns, Proteinase K, Reagents, Buffers, and Collection Tubes (2 ml)
SGD 555.00
登录 要查看您的账户定价。
QIAamp DSP DNA Mini Kit 旨在用于体外诊断用途。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 快速纯化高品质DNA
  • 产量高且稳定
  • 去除污染物和抑制剂

产品详情

QIAamp DSP DNA Mini Kit利用硅胶膜技术,从组织、拭子、脑脊液、血液、体液或尿液中洗下的细胞纯化核酸。离心柱操作流程无需机械匀浆,总的手工操作时间只需20分钟。

绩效

QIAamp DSP DNA Mini Kit使用QIAamp Mini离心柱从组织、拭子、CSF、血液、骨髓、体液、淋巴细胞和尿液中洗下的细胞中纯化核酸。DNA的产量和浓度取决于样本类型和洗脱体积。

原理

QIAamp DSP DNA Mini Kit可快速、便利的纯化总DNA。无需酚氯仿抽提。DNA特异性结合在QIAmp硅胶膜上,而污染物可流过。QIAamp DSP DNA Mini Kit可在20分钟内制备即用于下游应用的纯化DNA。

程序

QIAamp DSP DNA Mini Kit使用QIAamp Mini离心柱,操作流程有4个简单的步骤:裂解、结合、两次洗涤和洗脱。

使用QIAamp DSP DNA Mini Kit纯化DNA可在QIAcube全自动核酸纯化仪上自动完成。QIAamp DSP DNA Mini Kit含有QIAamp Mini离心柱,可使用离心机和真空底座处理(如QIAvac 24 Plus和VacConnectors及VacValves)。如果使用QIAamp DSP DNA Mini Kit在QIAcube全自动核酸纯化仪上自动操作,由于自动移液时的体积损失、蒸发和额外的试剂消耗,仪器处理的样品数可能少于50个。但使用QIAamp DSP DNA Mini Kit手工操作,可进行50次样品制备。

应用

QIAamp DSP DNA Mini Kit利用硅胶技术可从以下样本类型中纯化核酸:

  • 组织
  • 拭子
  • CSF
  • 全血、血浆、血清或白细胞层
  • 骨髓
  • 体液
  • 淋巴细胞 
  • 尿液中洗下的细胞

资源

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

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