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ipsogen BCR-ABL1 Mbcr Kit

采用real-time PCR对BCR-ABL Mbcr b2a2或b3a2转录本进行定量检测

S_3154_IpsogenBCR_s

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ipsogen BCR-ABL1 Mbcr Kit (24)

Cat. No. / ID:  670113

For 24 reactions: ABL Control Gene Standards, BCR-ABL Mbcr Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL Mbcr Fusion Gene
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 灵敏、准确的定量检测试剂
  • 符合EAC要求的标准化试剂,结果具有可重复性
  • 即用型解决方案,便利的工作流程

产品详情

ipsogen BCR-ABL1 Mbcr Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对BCR-ABL Mbcr b2a2或b3a2转录本在总RNA中相对于ABL对照基因的表达进行可靠、灵敏的定量检测。ipsogen BCR-ABL1 Mbcr IS-MMR Kit的技术参数和分析性能卓越,能够获得灵敏、准确、并符合国际标准的检测结果,可对结果进行主要分子反应(MMR)评估。

绩效

为确保高灵敏度,ipsogen BCR-ABL1 Mbcr Kit已经过优化,使用预稀释的质粒标准品和引物探针混合液,检测BCR-ABL Mbcr b2a2或b3a2转录本(参见 Accurate detection of BCR-ABL Mbcr standards Reliable detection of ABL plasmid standards)。
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原理

ipsogen BCR-ABL1 Mbcr Kit为即用型试剂盒,采用real-time PCR技术,检测BCR-ABL Mbcr b2a2或b3a2转录本。该试剂盒能够进行BCR-ABL Mbcr b2a2或b3a2转录本在总RNA中相对于ABL对照基因表达的相对定量。该试剂盒灵敏度高,特异性强,可重复性好。ipsogen BCR-ABL1 Mbcr Kit为Mbcr提供5个标准品稀释液,为ABL提供3个标准品稀释液。使用标准品能够实现准确的定量检测。该试剂盒提供用于检测BCR-ABL1 Mbcr基因和ABL对照基因的特异性引物探针混合液和梯度稀释的对照基因和融合基因DNA,符合欧洲抗癌协会(EAC)的推荐(参见 BCR-ABL Mbcr fusion gene transcript ABL control gene transcript)。
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程序

第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增cDNA。ipsogen BCR-ABL1 Mbcr Kit可对BCR-ABL Mbcr p210 b2a2或b3a2融合基因和ABL基因转录本进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

应用

ipsogen BCR-ABL1 Mbcr Kit能够对BCR-ABL Mbcr p210 b2a2或b3a2转录本相对于ABL对照基因的表达进行可靠、灵敏的定量检测。仅适用于科研。

辅助数据和图表

资源

试剂盒操作手册 (1)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
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