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ipsogen JAK2 MutaScreen Kits

采用real-time PCR对JAK2 V617F/G1849T突变进行半定量检测

Products

仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。
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ipsogen JAK2 MutaScreen Kit (10)

Cat. No. / ID:  673012

For 10 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, Primers and Probes Mix JAK2 wild-type and JAK2 V617F
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ipsogen JAK2 MutaScreen Kit (24)

Cat. No. / ID:  673013

For 24 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, Primers and Probes Mix JAK2 wild-type and JAK2 V617F
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ipsogen JAK2 MutaScreen RS Kit (19)

Cat. No. / ID:  673113

For 19 reactions: V617F Positive Control, V617F Negative Control, V617F Reference Scale, Primers and Probes Mix JAK2 wild-type and JAK2 V617F

特点

  • 可靠的半定量检测
  • 多重分析,无需优化
  • 即用型解决方案,便利的工作流程

产品详情

ipsogen JAK2 MutaScreen Kits仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对基因组DNA中的JAK2 V617F/G1849T突变进行灵敏、可靠的半定量检测。

绩效

为确保检测的准确性,ipsogen NJAK2 MutaScreen Kit已经过优化,利用DNA阳性和阴性对照,检测JAK2 V617F/G1849T突变(参见 Representative allelic discrimination experiments for qualitative detection of the JAK2 V617F/G1849T mutation in genomic DNA Representative allelic discrimination experiments for semi-quantitative detection of the JAK2 V617F/G1849T mutation in genomic DNA)。ipsogen JAK2 MutaScreen Kits的样本阳性DNA浓度检测阈限为2%,推荐的起始样本DNA量最少为25 ng。
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原理

ipsogen JAK2 MutaScreen Kits为即用型试剂盒,能够对基因组DNA中SNP导致的的JAK2 V617F/G1849T体细胞突变进行半定量检测分析(参见 JAK2 mutation site identification)。该试剂盒能够获得可靠、可重复性的结果。ipsogen JAK2 MutaScreen Kits提供JAK2 V617F的DNA检测临界样本(COS)、DNA阳性对照(PC)和阴性对照(NC)。使用检测临界样本和对照能够实现准确的基因分型分析。ipsogen JAK2 MutaScreen RS Kit提供V617F的参比。
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程序

第一步是抽提总DNA,并检测其量与品质。第二步是通过real-time PCR扩增DNA。ipsogen JAK2 MutaScreen Kits能够对JAK2 V617F/G1849T突变进行可靠的检测和半定量。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

应用

ipsogen JAK2 MutaScreen Kits能够对JAK2 V617F/G1849T突变进行可靠的检测和半定量,仅适用于科研。

辅助数据和图表

资源

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
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