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ipsogen JAK2 MutaSearch Kit

采用real-time PCR对JAK2 V617F/G1849T突变进行定量检测

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ipsogen JAK2 MutaSearch Kit (24)

Cat. No. / ID:  673813

For 24 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, 2 Primers and Probe Mixes JAK2 wild-type and JAK2 V617F
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 灵敏、准确的定量检测试剂
  • 能够检测1%临界值的阳性样本
  • 即用型解决方案,便利的工作流程

产品详情

ipsogen JAK2 MutaSearch Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对基因组DNA中的JAK2 V617F/G1849T突变进行灵敏、可靠的检测。

绩效

为确保检测的高灵敏度,ipsogen JAK2 MutaSearch Kit已经过优化,利用1%的DNA临界样本(COS)、阳性和阴性对照,检测JAK2 V617F/G1849T突变(参见 Accurate detection of JAK2 V617F mutation, 1% cut-off sample, and positive and negative controls Reliable detection of wild-type JAK2, 1% cut-off sample, and positive and negative controls)。推荐的起始样本DNA最少为25 ng。
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原理

ipsogen JAK2 MutaScreen Kits为即用型试剂盒,能够对基因组DNA中SNP导致的JAK2 V617F/G1849T体细胞突变进行检测分析(参见 JAK2 mutation site identification)。该试剂盒灵敏度高,可重复性强。ipsogen JAK2 MutaScreen Kits提供JAK2 V617F的DNA检测临界样本(COS)、DNA阳性对照(PC)和阴性对照(NC)。使用检测临界样本和对照能够实现准确的基因分型分析。
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程序

第一步是抽提总DNA,并检测其量与品质。第二步是通过real-time PCR扩增DNA。ipsogen JAK2 MutaSearch Kits能够对JAK2 V617F/G1849T突变进行灵敏的定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

应用

ipsogen JAK2 MutaSearch Kits能够对JAK2 V617F/G1849T突变进行灵敏、可靠的定量检测,仅适用于科研。

辅助数据和图表

资源

试剂盒操作手册 (1)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
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