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PyroMark Custom Assays

用于用户指定的DNA序列的焦磷酸测序分析

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PyroMark Custom Assay (200)

Cat. No. / ID:  978776

User-designed PCR and sequencing primers for Pyrosequencing analysis (200 reactions; tube format)
US$98.20
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PyroMark Custom Assays适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 针对用户指定的的引物序列的即用型试剂
  • 可在GeneGlobe便利的订购
  • 优化的引物浓度和品质
  • 采用QIAGEN专业的焦磷酸测序技术

Product Details

PyroMark Custom Assays是针对用户指定的的引物序列设计的即用型分析,可对任意DNA序列进行焦磷酸测序分析。

Principle

PyroMark Custom Assays使用户可灵活选择高品质的焦磷酸测序试剂盒,用于分析任意DNA序列。只需输入预制PCR和测序引物序列,即可获得即用型分析,且浓度经过优化。输入的引物序列可来自文献、以往项目的经验等,也可以使用PyroMark Assay Design Software 2.0设计并评估,确保实验成功。PyroMark Custom Assays与其他PyroMark产品线的产品,包括PCR引物和测序试剂,配合使用,可提高焦磷酸测序工作流程的效率,实现对任意基因组中的任意序列的分析。

优化的引物浓度

PyroMark Custom Assays中的PCR和测序引物皆为最佳浓度,使用便利,并提高了PCR的品质。与PyroMark PCR Kit中的优化试剂配套使用,可提高测序模板的产量和品质,进而提高分析的可靠性。新的PyroMark OneStep RT-PCR Kit可用于RNA样本,一次反应就可完成逆转录和模板扩增,优化产量并且分析结果可靠。

GeneGlobe的优势

QIAGEN的GeneGlobe Web门户是一站式在线数据库,可按照不同的基因或信号通路,选择多种多样的分析方法和技术。GeneGlobe具有最先进的搜索和设计技术、最新的序列信息和创新的技术。只需输入您感兴趣的基因或信号通路的名称,GeneGlobe将会为您展示在表观遗传学、基因表达、疾病的致病基因等领域如何优化您的研究(参见" Ordering through GeneGlobe")。

See figures

Procedure

在焦磷酸测序之前,从亚硫酸氢盐转化DNA获得荧光素标记的PCR产物。荧光素标记的PCR产物结合到覆盖链酶亲和素的Sepharose磁珠上。在PyroMark Vacuum Workstation上,将磁珠吸附到真空容器中,充分洗涤,获得适合测序的单链DNA。PyroMark Custom Assays使用户可灵活选择高品质的焦磷酸测序试剂盒,用于分析任意DNA序列。每个分析即含有产生荧光素标记的PCR产物所需的引物,也含有焦磷酸测序反应所需的引物。

Applications

焦磷酸测序技术对各个领域的研究应用至关重要。焦磷酸测序技术能够灵敏的分析遗传学和表观遗传学的变化,如检测病原体抗药性的产生,基因表达分析中表观遗传学DNA甲基化的作用、牲畜特异性表现型的遗传标记,以及法医检材样本中线粒体DNA的多态性。PyroMark Custom Assays为这些分析提供高品质的即用型引物。

Supporting data and figures

Resources

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