Looking for a quick way to design experiments?
Try the Workflow Configurator. A convenient tool to build experimental workflows and find products to match your needs.

artus CMV PCR Kits CE

使用real-time PCR技术定量检测巨细胞DNA

Products

artus CMV PCR Kits CE 旨在用于欧洲体外诊断用途。
Image

artus CMV RG PCR Kit (96) CE

Cat. No. / ID:   4503265

For 96 reactions on Rotor-Gene Q instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Image

artus CMV QS-RGQ Kit (24) CE

Cat. No. / ID:   4503363

For 24 reactions on the QIAsymphony RGQ: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Image

artus CMV LC PCR Kit (24) CE

Cat. No. / ID:   4503063

For 24 reactions on the LightCycler 1.1/1.2/1.5/2.0 Instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Image

artus CMV RG PCR Kit (24) CE

Cat. No. / ID:   4503263

For 24 reactions on Rotor-Gene Q instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Image

artus CMV LC PCR Kit (96) CE

Cat. No. / ID:   4503065

For 96 reactions on the LightCycler 1.1/1.2/1.5/2.0 Instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Image

artus CMV TM PCR Kit (24) CE

Cat. No. / ID:   4503163

For 24 reactions on the ABI PRISM 7000, 7700, 7900HT SDS: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Image

artus CMV TM PCR Kit (96) CE

Cat. No. / ID:   4503165

For 96 reactions on the ABI PRISM 7000, 7700, 7900HT SDS: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)

特点

  • 工作流程完全符合体外诊断CE认证(QIAsymphony RGQ)
  • 使用内参,具有高度可靠性
  • 检测度灵敏高,可达42.5拷贝/ml(QS-RGQ试剂盒)
  • 提供4个标准品,可准确定量病毒

产品详情

artus CMV PCR Kits是使用real-time PCR技术的即用型分子检测试剂盒。该试剂盒包含体外诊断中对巨细胞病毒DNA进行可靠定量检测的所有所需试剂,并已经过优化。artus CMV RG和TM PCR Kits使用手动样本制备和反应体系构建技术。artus CMV LC PCR Kit使用自动或手动样本制备和手动的反应体系构建技术。artus CMV QS-RGQ Kit可在QIAsymphony RGQ仪器上使用,从样本制备到病原体检测均为完全自动完成,不可进行手动反应体系构建。

绩效

为确保高灵敏度,artus CMV Kits已经过优化,可检测到低拷贝数的巨细胞病毒DNA。artus CMV QS-RGQ Kit在QIAsymphony RGQ上使用,对EDTA血浆样本进行分析的灵敏度为42.5拷贝/ml。

artus CMV RG PCR Kit and artus CMV QS-RGQ Kit性能特征
试剂盒  artus CMV RG PCR Kit artus CMV QS-RGQ Kit
已经过验证的适用样本类型 EDTA血浆 EDTA血浆或全血
分析灵敏度  57.1拷贝/ml 42.5拷贝/ml(血浆)
线性范围  n.d. 79.4至1.00 x 108 拷贝/ml(血浆)
n.d.: 未确定。

原理

artus CMV Kits的原理是使用real-time RT-PCR技术,扩增和实时检测巨细胞病毒基因组中一段特定序列。该试剂盒特异性强、灵敏度高(参见"Highly sensitive detection of CMV DNA"),且重复性好。

每个artus CMV Kit都包含4个巨细胞病毒标准品(参见"Reliable quantitation of CMV load")。使用这些标准品可确保准确定量病毒。此外,该试剂盒还含有异源扩增系统,即内参,可识别PCR抑制剂。内参(IC)可在PCR中通过不同的荧光通道检测,而不会降低巨细胞病毒的检测灵敏度。

artus CMV RG PCR Kit和artus CMV QS-RGQ Kit的规格
试剂盒  artus CMV RG PCR Kit artus CMV QS-RGQ Kit
经过验证的适用样本类型  EDTA血浆  EDTA血浆或全血
扩增子  即刻早期基因的一段长为105 bp的序列  即刻早期基因的一段长为105 bp的序列

程序

artus CMV PCR Kits包含对巨细胞病毒DNA进行可靠定量检测的所有所需试剂,并已经过优化。只需在即用型PCR预混液和Mg溶液中加入模板DNA,即可按照试剂盒操作手册中的优化程序,在real-time PCR仪上开始反应。

多个试剂盒的反应温度相近,一次检测可进行多个分析

artus CMV RG PCR Kit和artus CMV QS-RGQ Kit的温度范围与artus RG PCR Kits和artus QS-RGQ Kits用于检测BK病毒、EBV、HSV-1/2和VZV的试剂盒的温度范围相同。因此,这些PCR分析可以在一次操作中同时进行。

完整的自动化检测体系,覆盖样本制备到巨细胞病毒检测

QIAsymphony RGQ的巨细胞病毒检测解决方案包括:使用QIAsymphony SP进行样本制备,使用QIAsymphony AS进行反应体系构建,并在Rotor-Gene Q实时荧光定量PCR分析仪上使用artus CMV QS-RGQ Kit进行检测。这一体系不仅可可靠检测病原体,而且完全符合体外诊断CE的要求(参见"Integrated QIAsymphony RGQ system for CMV detection")。

如使用artus CMV LC、RG或TM PCR Kits,推荐用于纯化DNA的产品

artus RG和TM PCR Kits已经过验证,可与QIAamp DSP Virus Kit共同使用。QIAamp DSP Virus Kit或EZ1 DSP Virus Kit则可用于artus CMV LC PCR Kit 。artus CMV LC PCR Kit的经过体外诊断CE认证的产品为EASYartus PCR Kits,可与用于自动纯化病毒核酸的EZ1 DSP Virus Kit共同使用。

应用

artus CMV RG、LC和TM PCR Kits可快速、灵敏、准确的定量检测EDTA血浆中的巨细胞病毒DNA。这些试剂盒适用于ABI PRISM 7000、7700和7900HT SDS,LightCycler 1.1/1.2/1.5/2.0 Instruments或Rotor-Gene Q实时荧光定量PCR分析仪。

artus CMV QS-RGQ Kit已经过验证,可检测EDTA血浆或全血,并适用于QIAsymphony RGQ仪器,整个工作流程覆盖样本制备到巨细胞病毒DNA定量检测,完全符合体外诊断CE要求。

资源

实验方案软件 (1)
For use with QIAsymphony SP/AS instruments (software version 4.0)
仪器技术参数 (1)
Information document for software version 4.0
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
//dev-homepage/applications/digital-pcr/promotions/registration/search/products?query=QIAGEN%20Plasmid%20Mini%20Kit%20(100/search3/products?query=dna/search3/products/dev-search/products?query=dna/dev-search/products/product-categories/discovery-and-translational-research/genomic-services/product-categories/discovery-and-translational-research/genomic-services?disable-wfc=true&disable-dtm=true/products/products/diagnostics-and-clinical-research/sample-processing/allprep-rnaprotein-kit/products/diagnostics-and-clinical-research/sample-processing/allprep-rnaprotein-kit/products/discovery-and-translational-research/dna-rna-purification/dna-purification/genomic-dna/blood-and-cell-culture-dna-mini-kit/products/discovery-and-translational-research/pcr-qpcr/pcr-enzymes-and-kits/hifidelity-long-range-and-other-pcr/ucp-hifidelity-pcr-kit/products/discovery-and-translational-research/lab-essentials/buffers-reagents/maxtract-high-density/products/discovery-and-translational-research/lab-essentials/buffers-reagents/maxtract-high-density?catno=129056/products/discovery-and-translational-research/lab-essentials/buffers-reagents/maxtract-high-density?catno=129065/products/diagnostics-and-clinical-research/sample-processing/allprep-rnaprotein-kit/BAD_URL/product-categories/discovery-and-translational-research/genomic-services/BAD_URL/products?cmpid=1234&intcmp=456/products?cmpid=asdf&intcmp=qwertysds-searchpromotionsknowledge-and-support/resourcesapplications/enzymes/tools-and-calculatorsapplications/enzymes/tools-and-calculators/ligation-calculator