Ligation Calculator
This tool will calculate the mass of insert required at several molar insert:vector ratios in the range needed for typical ligation reactions.
Formula
required mass insert (g) = desired insert/vector molar ratio x mass of vector (g) x ratio of insert to vector lengths (p) test
Read more
Related products
CAT NO./ID L6010L
T3 DNA Ligase (900,000 U)
900,000 U of T3 DNA Ligase (3,000,000 U/mL) and 2x Rapid Ligation Buffer
CAT NO./ID L6030-HC-L
T4 DNA Ligase (Rapid)
240,000 U of T4 DNA Ligase (600,000 U/mL) 2x Rapid Ligation Buffer and 10x T4 DNA Ligase Buffer
CAT NO./ID L6020L
T7 DNA Ligase (900,000 U)
900,000 U of T7 DNA Ligase (3,000,000 U/mL) and 2x Rapid Ligation Buffer
CAT NO./ID P7050L
DNA Polymerase I (5000 U)
5000 U of DNA Polymerase I (10,000 U/mL) and 10x Blue Buffer. Storage temperature: –25°C to –15°C
CAT NO./ID 12123 icon_0299_cc_gen_leaves-s
QIAGEN Plasmid Mini Kit (25).
25 QIAGEN-tip 20, Reagents, Buffers. h j
"Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod
tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim
veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea
commodo consequat. Duis aute irure dolor in reprehenderit in voluptate
velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint
occaecat cupidatat non proident, sunt in culpa qui officia deserunt
mollit anim id est laborum."