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Enzymes for Molecular Biology

Enzymes for Assays & Detection

Footprints and fragments, comets and maps, TUNELs and transcripts

Enzymes give you the power to discover sites where proteins bind to DNA, to determine transcript abundance, reveal single base substitutions, map exons, visualize apoptosis and measure DNA damage in cells. 

Enzyme footprinting characterizes DNA-protein interactions

DNA breaks indicate apoptosis and genotoxicity

RNase A digestion locates base substitutions and maps transcripts

The capacity of enzymes to synthesize or digest nucleic acids, cleave them at specific sites or excise individual bases from a chain makes it possible to apply these enzyme attributes to answer questions through detection, assay and analysis.

Enzyme   Activity Assay
79254
RNase-free DNase
(1500 Kunitz units)
Endonuclease that nonspecifically cleaves DNA to release di-, tri-
and oligonucleotide products with 5’-phosphorylated and
3’-hydroxylated ends; acts on ssDNA and dsDNA, chromatin
and RNA:DNA hybrids 		DNA-protein interaction analysis (Footprinting assay for
DNA-binding proteins)
X8020L
Exonuclease III		 Coming soon Exonuclease that excises bases from duplex DNA in 3’→5’
direction 		DNA-protein interaction analysis 
(Footprinting assay for DNA-binding proteins)
19101
RNase A
Endoribonuclease that degrades ssRNA at C and U residues
  • Determining relative or absolute transcript abundance
  • Mapping mRNA termini and intron/exon boundaries
    (RNase protection assay)
  • Mapping single-base mutations in DNA or RNA via
    cleavage at a single base mismatch in an RNA:DNA hybrid
P7260L
T7 DNA Polymerase		 Coming soon  DNA polymerase with high rate of synthesis and replication
fidelity; a two-subunit protein with a polymerase domain and
a processivity factor (E. coli thioredoxin)		  In situ labeling of DNA damage (Apoptosis assay)
Y9080L
Endonuclease VIII		 Coming soon Endonuclease VIII functions as both an N-glycosylase
(by excising oxidative base lesions) and an AP lyase (by
subsequently cleaving the phosphodiester backbone), leaving
terminal phosphates at the 5’ and 3’ ends; participates in base
excision repair of oxidatively generated DNA damage 		Measurement of DNA damage via single cell gel
electrophoresis (Comet assay)

FAQs about enzymes for assays and detection

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