The importance of rRNA removal in RNA-seq optimization

QIAseq FastSelect enables high-quality RNA-seq from mixed human and bacterial samples

Dr. Marie Adams, Genomics Core Director at Van Andel Institute, discusses how QIAseq FastSelect technology helped conquer the challenges of generating high-quality RNA-seq data for human and bacterial co-expression studies from the same RNA sample.

QIAseq FastSelect helps pave the way towards more successful cancer treatment

“QIAseq FastSelect has been phenomenal with the RNA sequencing in my project. The RNA was degraded and almost unusable, but QIAseq FastSelect really removes the ribosomal RNA in these degraded samples and has improved our sequencing libraries”.

Brandon Mistrella, University of Houston's Department of Biology and Biochemistry

Cancer researchers at the University of Houston routinely encountered challenges associated with RNA degradation when working with FFPE samples. The presence of unwanted RNA species such as ribosomal RNA and globin mRNA in the low-yield RNA sample further complicated RNA sequencing. Read how QIAseq FastSelect transformed their research, allowing them to retrieve useful RNA-seq data from FFPE RNA.

QIAseq FastSelect enables construction of RNA-seq libraries from low-quality FFPE RNA

“The workflow is very simple and fast. I would say that with poor-quality samples and with the limited quantity of those samples, I wasn’t expecting the results we obtained.”

Dr. Fabienne Desmots-Loyer, Hematology researcher, CHU – Hospital Pontchaillou, Rennes, France

Due to high levels of degradation and poor yields, working with RNA from FFPE samples can be tough. Read the story of Dr. Fabienne Desmots-Loyer at the Hospital Rennes and find out how QIAseq FastSelect saved the day by providing high-quality libraries from FFPE cancer samples previously rejected for processing by two commercial labs due to high levels of RNA degradation and low yields.

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Dramatically faster and easier rRNA removal

While alternative rRNA removal methods take several hours, require multiple steps, work solely on unfragmented RNA or simply don’t remove enough rRNA, with the FastSelect method you benefit from a dramatically faster and more convenient workflow – and exceptional results even with FFPE and fragmented RNA samples.

Rely on QIAGEN Genomic Services for your research needs

Take advantage of QIAGEN Genomic Services to accelerate your research. From library prep and sequencing to data analysis and interpretation, our in-house experts use trusted QIAseq NGS technologies and QIAGEN Digital Insights software to deliver high-quality results you can rely on.