PCR

Real-time qPCR

Applications

SARS-CoV-2 epidemiology

Reliable genotyping of virus isolates is essential to understand the evolution, virulence and transmission of SARS-CoV-2 and emerging variants. Moreover, during the respiratory virus season, when there is a significant overlap between SARS-CoV-2, flu A/B and RSV A/B symptoms, it is critical to accurately discriminate between the co-circulating viruses by multiplex, real-time PCR. Discover a robust, same-day genotyping approach and a simple, cost-effective multiplexing strategy to match your research goals.

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COVID
PCR

Gene expression analysis

Analyzing gene expression by quantifying the corresponding mRNA or lncRNA is critical to many areas such as cancer and disease research and biomarker identification. For quantification of known targets, reverse transcription qPCR (RT-qPCR) remains the gold standard method, providing fast analysis from just a few to many genes

Our gene expression products enable sensitive quantification with any type of RNA or transcript abundance to deliver unbiased gene expression profiles and get you to your insights faster.

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Female scientists

miRNA quantification and profiling

MicroRNAs (miRNAs) have emerged as potential biomarkers for numerous diseases and biological processes, such as cancer, heart disease, nervous system function, hemostasis and DNA repair.

Quantitative PCR is the tool of choice for analyzing known miRNA targets and offers several benefits over NGS. qPCR is fast, simple and inexpensive for most labs to set up and provides extremely sensitive analyses from low amounts of input RNA. It can be easily scaled to various research needs, from screening and profiling, to validation of NGS studies and verification of results from functional studies.

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Microbial analysis

Standard PCR kits and reagents can introduce tiny amounts of contaminating nucleic acids into your experiments, jeopardizing results and conclusions.

Designed with microbiome and sensitive microbial applications in mind (e.g., microbial ID, food surveillance, pathogen detection, QC or residual DNA testing, and microbial-host interactions), our new UltraClean Production (UCP) real-time PCR kits bring greater precision to your experiments and confidence to your results.

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UCP

Detecting microbial contamination

Up to 70% of mammalian cell cultures used to publish work are unknowingly contaminated by bacteria, viruses, or parasites. One company has set the course to help researchers and scientists detect contamination and obtain valid results. Perfectus Biomed has built a microbiology lab from the ground up using QIAGEN PCR technology to ensure valid results, cell line detection, and no contamination.

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dPCR
PCR

DNA quantitation

Quantitation of DNA targets plays an important role in many research areas: from detection of genetically modified organisms (GMO) in food to the analysis of genetic variations such as single nucleotide polymorphisms (SNPs), copy number variations (CNV) and somatic mutations.

Our selection of QuantiNova Real-Time PCR Kits help remove the experimental variation and human errors from your qPCR workflow to deliver precise and reproducible quantitation.

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Analyzing copy number variation? Go digital.
Digital PCR can easily detect small fold-change differences such as less than a 1.2-fold change in CNV from four to five copies, without requiring a standard curve.
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qPCR Chemistry

qPCR

Discover the benefits of QuantiNova 

QuantiNova kits represent today’s most advanced portfolio of real-time PCR and RT-PCR kits for gene expression analysis, gene quantification and pathogen detection. A unique combination of in-process controls removes variables and minimizes human error, so your experiments are bound to work. Simple and fast procedures increase your efficiency, saving you time and money. Plus, flexibility in detection chemistry and full real-time cycler compatibility mean there’s no need to compromise.

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Experience the benefits of QuantiNova ​

Looking for one of our other qPCR solutions?

Does your project have unique requirements? Our complete range of real-time PCR solutions has you covered. Check out these other offerings or contact one of our experts to find the best tools for your research.

QuantiTect

FastLane

Microbial DNA qPCR

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Experiment Design

Ensuring high-quality, reproducible results

qPCR is a ubiquitous research tool, but improperly designed experiments will generate questionable findings. The MIQE guidelines define the standards for designing and reporting qPCR experiments.

What makes a good qPCR assay? High accuracy, dynamic range, reliability, reproducibility, sensitivity and specificity. The primer design algorithm and the reaction buffer conditions must work together to provide the best results. Designing assays without fully understanding the critical design aspects can unknowingly compromise the results.

We can help remove the guesswork from your experiments. Our broad selection of qPCR kits and an unparalleled choice of predesigned assays and panels along the option to design your own using our advanced algorithms ensure quality qPCR results time and again.

Consider these factors for useful and meaningful results

  • icon to do list
    Experiment design
    Define the problem, form hypotheses and cover all factors. Consider controls, type and number of replicates, sample handling and sources of variation.
  • Sample management
    Sample management
    mRNA profiles can quickly change after tissue/blood collection. Standardize sampling techniques and timepoints and use a reliable RNA stabilization method.
  • RNA isolation / gDNA removal
    RNA isolation / gDNA removal
    gDNA contamination leads to inaccurate quantification. Avoid unwanted DNA detection by removing gDNA or using specially designed primers.
  • Reference gene selection
    Reference gene selection
    Choose appropriate reference genes to avoid bias. Consider using 2–5 validated, stably-expressed reference genes for normalization.
  • icon with monitor
    Data analysis
    Enable transparent data exchange by using real-time cyclers with built-in RDML export capabilities. Ensure accurate data processing and interpretation.

Resources and Tools

Latest – webinars and other tools

Guides and tips for great results​

Real-Time Cyclers

Raise your qPCR workflow to the next level

It is increasingly challenging to find the right real-time PCR cycler for your laboratory’s application requirements. For best results, you need low operating costs, high throughput, the ability to multiplex with fast analysis times and reliable reproducibility. As the list of features and specifications grows, an understanding of some of the key performance benefits such as precision, uniformity, dynamic range and resolution becomes critical.

Choose the instrument that suits your needs from our portfolio of real-time PCR cyclers.

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